产品编号:A270
产品描述:Anti-Human Akt1
反应种属:mouse, human, rat
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5700.00
订购:
说明书:
Rabbit Anti-Human Akt1
Purified No Carrier Protein
(Protein Kinase B/PKB)
Polyclonal Antibody
Prod. No.: A270
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Akt1 recognizes an epitope neat the N-terminus of human, mouse and rat Akt1. This polyclonal antibody was purified using affinity chromatography.
Background:
Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt,1 is part of the phosphatidyl 3’-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival.2 Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2.3 The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad.4 Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Akt1 Blocking Peptide (Leinco Prod. No.: A279)
Blocking Peptide
Akt1 Blocking Peptide (Leinco Prod. No.: A279)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human, mouse and rat Akt1 by Western blot analysis at a concentration of 1 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Liver Tissue Lysate (Leinco Prod. No.: H1408).
References
1. Bellacosa, A. et al. (1991) Science 254:274
2. Song, G. et al. (2005) J. Cell. Mol. Med. 9:59
3. Alessi, D. R. et al. (1996) EMBO J. 15:6541
4. Nave, B. T. et al. (1999) Biochem. J. 344:427
Related Products
Akt1 Blocking Peptide (Leinco Prod. No.: A279)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Liver Tissue Lysate (Leinco Prod. No.: H1408)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
