产品编号:A184
产品描述:Anti-Human ADAM10(CT)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human ADAM10
Purified No Carrier Protein
(KUZ, MADM)
Polyclonal Antibody
Prod. No.: A184
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human ADAM10 recognizes an epitope near the C-terminus of Human ADAM10. This polyclonal antibody was purified using affinity chromatography.
Background:
Proinflammatory cytokine tumor necrosis factor-α (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta.1,2,3 The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively.1,2,4 ADAM10 mRNA is expressed in a variety of human and bovine tissues.1,4
Known Species Cross Reactivity: Hu+
Immunogen
ADAM10 (CT) Blocking Peptide (Leinco Prod. No.: A169)
Blocking Peptide
ADAM10 (CT) Blocking Peptide (Leinco Prod. No.: A169)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentraion of 2 µg/ml. The positive control for IHC is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
Western Blotting: This antibody can be used to detect Human ADAM10 by Western blot analysis at a dilution of 1:500 to 1:2000 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumor necrosis factor-α-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 1997;272:24588-93
2. Pan D, Rubin GM. Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997;90:271-80
3. Qi H, Rand MD, Wu X, Sestan N, Wang W, Rakic P, Xu T, Artavanis-Tsakonas S. Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999;283:91-4
Related Products
ADAM10 (CT) Blocking Peptide (Leinco Prod. No.: A169)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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