产品编号:A187
产品描述:Anti-Human Act1(NT)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human Act1
Purified No Carrier Protein
(CIKS)
Polyclonal Antibody
Prod. No.: A187
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Act1 recognizes an epitope near the N-terminus of Human Act1. This polyclonal antibody was purified using affinity chromatography.
Background:
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB associates with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-κB activator 1).1,2 CIKS directly interacts with IKKγ. CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.
Known Species Cross Reactivity: Hu+
Immunogen
Act1 (NT) Blocking Peptide (Leinco Prod. No.: A188)
Blocking Peptide
Act1 (NT) Blocking Peptide (Leinco Prod. No.: A188)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human Act1 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Placenta Tissue Lysate (Leinco Prod. No.: P165).
References
1. Leonardi, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97(19):10494
2. Li, X. et al. (2000) Proc. Natl. Acad. Sci. USA 97(19):10489
Related Products
Act1 (NT) Blocking Peptide (Leinco Prod. No.: A188)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Placenta Tissue Lysate (Leinco Prod. No.: P165)
Act1 (CT) Blocking Peptide (Leinco Prod. No.: A190)
Anti-Human Act1 (CT) (Leinco Prod. No.: A189)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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