产品编号:A148
产品描述:Anti-Human Acinus(C-Terminus 2)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.1mg
供应商:leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human Acinus
Purified No Carrier Protein
(Apoptotic Chromatin Condensation Inducer in the Nucleus)
Polyclonal Antibody
Prod. No.: A148
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Acinus (CT-2) recognizes an epitope near the C-terminus of Human Acinus (CT-2). This polyclonal antibody was purified using immobilized peptide affinity chromatography.
Background:
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’.2
Known Species Cross Reactivity: Hu+
Immunogen
Acinus (C-Terminus 2) Blocking Peptide (Leinco Prod. No.: A166)
Blocking Peptide
Acinus (C-Terminus 2) Blocking Peptide (Leinco Prod. No.: A166)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentraion of 0.5 µg/ml. The positive control for IHC is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
Western Blotting: This antibody can be used to detect Human Acinus (CT-2) by Western blot analysis at a concentration of 0.25-0.5 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
Related Products
Acinus (C-Terminus 2) Blocking Peptide (Leinco Prod. No.: A166)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Acinus (C-Terminus-1) Blocking Peptide (Leinco Prod. No.: A165)
Anti-Human Acinus (C--Terminus 1) (Leinco Prod. No.: A147)
PN:
Acinus (N-Terminus) Blocking Peptide (Leinco Prod. No.: A168)
Anti-Human Acinus (Intermediate Domain) (Leinco Prod. No.: A149)
Acinus (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: A167)
Anti-Human Acinus (N-Terminus) (Leinco Prod. No.: A150)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
