产品编号:N100
产品描述:Anti-Human Nucleolus
反应种属:human
实验方法:IHC
标记:
规格:2.0 ml
供应商:leinco
价格(RMB):3300.00
订购:
说明书:
Anti-Human Nucleolus
Tissue Culture Supernatant
Monoclonal Antibody
Prod. No.: N100
Clone: AE-3
Isotype: Mouse IgG1
Pkg. Size: 2.0 ml
Description
Specificity:
Mouse Anti-Human Nucleolus (Clone AE-3) recognizes a specific antigen found in the nucleolus of human cless. The sequence of the epitope has not been mapped.
Background:
In cell biology, the nucleolus (plural nucleoli) is a sub-organelle of the cell nucleus, which itself is an organelle. A main function of the nucleolus is the production and assembly of ribosome components. The nucleolus is roughly spherical, and is surrounded by a layer of condensed chromatin. No membrane separates the nucleolus from the nucleoplasm. Nucleoli are made of protein and ribosomal DNA (rDNA) sequences of chromosomes. The rDNA is a fundamental component since it serves as the template for transcription of the ribosomal RNA (rRNA) for inclusions in new ribosomes. Most plant and animal cells have one or more nucleoli, but some cell types do not have any. Since nucleoli carry out the production and maturation of ribosomes, large numbers of ribosomes are found inside them. In addition to ribosome biogenesis, nucleoli are believed to have other roles in cellular activity. Nucleoli fragment during cell reproduction (they can no longer be seen in metaphase of mitosis, when the chromosome copies separate). After the daughter cells complete their separation, the fragments of nucleoli fuse together around the nucleolus organizer regions (NORs) of the chromosomes.
Host Species
Mouse
Formulation
Anti-Human Nucleolus is supplied in filtered tissue supernatant (RPMI-1640) pH-7.2 containig 0.09% sodium azide.
Storage and Stability
For long term storage freeze working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.)
IHC (NBF/Par.): Monoclonal AE-3 may be used with no dilution required to stain nucleoli in cell or tissue preparations and can be used to identify nucleoli in subcellular fractions. We highly recommend fixing cells or tissue sections with 2.0% paraformaldehyde. Tonsil is the recommended positive control tissue.
Products are for research use only. Not for use in diagnostic or therapeutic procedures
