产品编号:N102
产品描述:Anti-Human Neurofilament (70 kD)
反应种属:human
实验方法:IHC, WB
标记:
规格:0.125 mg
供应商:leinco
价格(RMB):3465.00
订购:
说明书:
Anti-Human Neurofilament (70 kD)
Purified
Monoclonal Antibody
Prod. No.: N102
Clone: 8A1
Isotype: Mouse IgG1
Conc.: 0.5 mg/ml
Pkg. Size: 0.125 mg
Storage: 2-8°C
Description
Specificity:
Anti-Neurofilament (70 kDa) recognizes the small subunit of the neurofilament triplet.
Antigen Distribution:
The 70 kDa neurofilament antigen is present in neurons and tumor cells of neuronal origin.
Background:
The family of proteins making intermediate filaments is divided into 5 major classes, the keratins forming the classes I and II. The neurofilament subunits occupy the class IV family of intermediate filaments, and was originally thought to contain only three proteins named NF-L, NF-M and NF-H. These names come from the apparent molecular weight of the mammalian subunits on SDS-PAGE:
- the light or lowest (NF-L) runs at 68-70kDa
- the medium or middle (NF-M) runs at about 145-160kDa
- the heavy or highest (NF-H) runs at 200-220kDa
The SDS-PAGE molecular weights vary between mammalian species, with larger species usually having larger proteins. Neurofilaments are found in vertebrate neurons in especially high concentrations along the axons, where they appear to regulate axonal diameter.
In the adult mammal neurofilament subunit proteins coassemble in vivo, forming a heteropolymer that contain NF-L plus NF-M or NF-H. The NF-H and NF-M proteins have lengthy C-terminal tail domains that appear to control the spacing between neighboring filaments, generating aligned arrays with a fairly uniform interfilament spacing.
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human Neurofilament (70 kD) this monoclonal antibody can be used at a concentration of 0.5 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
Guillemot, F. et al. (1992) Development 114:743
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Neurofilament (140-160 kD) (Leinco Prod. No.: N103)
Anti-Neurofilament (200 kD) (Leinco Prod. No.: N104)
Anti-Neurofilament-P (200 kD Phosphorylated) (Leinco Prod. No.: N105)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
