产品编号:C290
产品描述:Anti-Human CD57
反应种属:human
实验方法:IHC, FC, WB
标记:
规格:200ug
供应商:leinco
价格(RMB):5385.00
订购:
说明书:
Anti-Human CD57
R-phycoerythrin (R-PE)
(HNK-1, Natural Killer Cells)
Monoclonal Antibody
Prod. No.: C290
Clone: VC1.1
Isotype: Mouse IgM
Conc.: 0.1 mg/ml
Pkg. Size: 50 µg, 100 µg, 200 µg
Storage: 2-8°C
Description
Specificity:
Mouse Anti-Human CD57 (Clone VC1.1) recognizes Human CD57. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-CD57 recognizes a (Mr 110 kD) carbohydrate molecule on myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG).1 This antibody blocks binding of HNK-1 (Leu. 7) antibody.3
Antigen Distribution:
The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.
Background:
Anti-CD57 may be used in flow cytometry, Western blotting and immunohistology on formalin fixed paraffin embedded tissue sections. The antibody is useful for enumeration of NK subsets and T-lymphocyte subsets, studies of neural tissue, malignant tissues and AIDS-related complex.
Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.
Host Species
Mouse
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
IHC (NBF/Par.), FC, WB
FC: This antibody can be used at a concentration of ≤1 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
References
1. Barnstable, C. et al. (1995) Brain Research 559:118
2. Leukocyte Typing V (1995) Oxford University Press: Code No. NK67
3. Arimatsu, Y. et al. (1987) J. Neuroscience 7:1250
4. Abo, T., and Balch, C.M. et al. (1981) J. Immunol. 127:1024
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Anti-Human CD57/HNK-1 (Natural Killer Cells)-Allophycocyanin (APC) (Leinco Prod. No.: C325)
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Monoclonal Antibodies for Neuroscience
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
