产品编号:C288
产品描述:Anti-Human CD57/HNK-1 Biotin
反应种属:human
实验方法:IHC, FC, WB
标记:
规格:200ug
供应商:leinco
价格(RMB):4350.00
订购:
说明书:
Anti-Human CD57
Biotin
(HNK-1, Natural Killer Cells)
Monoclonal Antibody
Prod. No.: C288
Clone: VC1.1
Isotype: Mouse IgM
Conc.: 0.1 mg/ml
Pkg. Size: 50 µg, 100 µg, 200 µg
Storage: 2-8°C
Description
Specificity:
Mouse Anti-Human CD57 (Clone VC1.1) recognizes Human CD57. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-CD57 recognizes a (Mr 110 kD) carbohydrate molecule on myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG).1 This antibody blocks binding of HNK-1 (Leu. 7) antibody.3
Antigen Distribution:
The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.
Background:
Anti-CD57 may be used in flow cytometry, Western blotting and immunohistology on formalin fixed paraffin embedded tissue sections. The antibody is useful for enumeration of NK subsets and T-lymphocyte subsets, studies of neural tissue, malignant tissues and AIDS-related complex.
Host Species
Mouse
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
IHC (NBF/Par.), FC, WB
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD57. A suggested method would be to stain cells expressing CD57 with Anti-Human CD57 at ≤1 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104).
References
1. Barnstable, C. et al. (1995) Brain Research 559:118
2. Leukocyte Typing V (1995) Oxford University Press: Code No. NK67
3. Arimatsu, Y. et al. (1987) J. Neuroscience 7:1250
4. Abo, T., and Balch, C.M. et al. (1981) J. Immunol. 127:1024
Related Products
UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104)
Anti-Human CD57/HNK-1 (Natural Killer Cells)-Fluorescein (FITC) (Leinco Prod. No.: C289)
Anti-Human CD57/HNK-1 (Natural Killer Cells)-R-phycoerythrin (R-PE) (Leinco Prod. No.: C290)
Anti-Human CD57/HNK-1 (Natural Killer Cells)-Allophycocyanin (APC) (Leinco Prod. No.: C325)
Anti-Human CD57/HNK-1 (Natural Killer Cells) Purified (Leinco Prod. No.: C287)
UltraAvadin™ Reagents
Streptavadin Reagents
SurfaceTag™ Human CD Markers
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
