产品编号:C175
产品描述:Anti-Human CD56 (NCAM) Purified
反应种属:human
实验方法:FC, WB
标记:
规格:200ug
供应商:leinco
价格(RMB):2835.00
订购:
说明书:
Anti-Human CD56
Purified
(NCAM)
Monoclonal Antibody
Prod. No.: C175
Clone: ERIC-1
Isotype: Mouse IgG1
Conc.: 0.1 mg/ml
Pkg. Size: 50 µg, 100 µg, 200 µg
Description
Specificity:
Mouse Anti-Human CD56 (Clone ERIC-1) recognizes Human CD56. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM).1
Antigen Distribution:
The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle1 and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20°C) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes.
Background:
Anti-Human CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested.1
Host Species
Mouse
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC, WB
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD56. A suggested method would be to stain cells expressing CD56 with Anti-Human CD56 at ≤1 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat F(ab')2 Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M100).
References
1. Bourne, K. et al. (1991) J. of Neuro-Oncology 10:111
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Monoclonal Antibodies for Neuroscience
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
