产品编号:T124
产品描述:Anti β-Tubulin Purified
反应种属:mouse, human, rat, rabbit
实验方法:IHC, WB
标记:
规格:0.125 mg
供应商:leinco
价格(RMB):3465.00
订购:
说明书:
Anti-Human β-Tubulin
Purified
Monoclonal Antibody
Prod. No.: T124
Clone: 2.1
Isotype: Mouse IgG1
Conc.: 0.5 mg/ml
Pkg. Size: 0.125 mg
Storage: 2-8°C
Description
Specificity:
Mouse Anti-Human β-Tubulin (Clone 2.1) recognizes an epitope on β-Tubulin between amino acids 281-446. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
β-tubulin is present in all nucleated eukaryotic cells. The antigen recognized by this antibody is present in most all species of living organisms.
Background:
A Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After being incorporated into the microtubule, the bound molecule of GTP will hydrolize into GDP. Although both subunits bind GTP, only the β-subunit has GTPase activity; that is, β-tubulin can hydrolize GTP to GDP whereas α-tubulin cannot. Whether the β-tubulin member of the tubulin dimer is bound to GTP or GDP influences the stability of the dimer in the microtubule. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart; thus, this GTP cycle is essential for the dynamic instability of the microtubule.
Known Species Cross Reactivity: Ms+, Hu+, Rt+, Rb+
Host Species
Mouse
Immunogen
Purified Rat Brain Tubulin
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentraion of 1.0-2.0 µg/ml.
Western Blotting: To detect Human β-Tubulin by Western blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as M1087.
References
1. Gozes, I. and Barnstable, C. (1982) Proc. Natl. Acad. Sci. 79:2579
2. Southwick, P.L. et al. (1990) Cytomety 11:418
3. Matthes, T. et al. (1988) J. Immunol 141:3135
Related Products
Anti β-Tubulin (C-Terminus) (Leinco Prod. No.: T123)
Monoclonal Antibodies To Subcellular Organelles
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
