产品编号:H119
产品描述:Anti-Human LH-β
反应种属:human
实验方法:ELISA
标记:
规格:1.0 ml
供应商:leinco
价格(RMB):2670.00
订购:
说明书:
Anti-Human Luteinizing Hormone Beta (LH-β)
Horseradish Peroxidase (HRPO)
Monoclonal Antibody
Prod. No.: H119
Clone: 196
Isotype: Mouse IgG
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 ml
Description
Specificity:
Mouse Anti-Human Luteinizing Hormone Beta (LH=β) (Clone 196) recognizes Human Luteinizing Hormone Beta. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Luteinizing hormone (LH) is a hormone synthesized and secreted by gonadotropes in the anterior lobe of the pituitary gland. In concert with the other pituitary gonadotropin follicle stimulating hormone (FSH) it is necessary for proper reproductive function. In the female, an acute rise of LH – the LH surge – triggers ovulation. In the male, where LH had also been called Interstitial Cell Stimulating Hormone (ICSH), it stimulates Leydig cell production of testosterone. LH is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it; two of these make the full, functional protein. Its structure is similar to the other glycoproteins, FSH, TSH, and hCG. The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two disulfide bridges. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. LH has a beta subunit of 121 amino acids (LHB) that confers its specific biologic action and is responsible for interaction with the LH receptor. This beta subunit contains the same amino acids in sequence as the beta sub unit of hCG and both stimulate the same receptor, however, the hCG beta subunit contains an additional 24 amino acids, and both hormones differ in the composition of their sugar moieties. The different composition of these oligosaccharides affects bioactivity and speed of degradation. The biologic half-life of LH is 20 minutes, shorter than that of FSH (3-4 hours) or hCG (24 hours).
Reporter Molecule:
Horseradish Peroxidase (HRPO) is an enzyme purified from horseradish root with a molecular weight of approximately 44 kDa.
Affinity:
4 X 1010
Host Species
Mouse
Immunogen
Purified Recombinant Human LH=β (>98%)
Formulation
This horseradish peroxidase conjugate is supplied in a specially formulated stabilization diluent.(Leinco Prod. No.: S509)
Cross Reactivity
Paired with Leinco Prod. No. F103 Clone No. 176: LH 100%, BhCG < 1%, ihCG 57%, FSH < 1%, TSH < 1%. Paired with Leinco Prod. No. C102: Clone No. 151 LH 100%, BhCG > 40%, ihCG > 40%, FSH < 1%, TSH < 1%
Storage and Stability
Stable for at least one year when stored at 2°C - 8°C. Do not freeze.
Reported Applications
ELISA Det
ELISA Detection: This conjugate can be used as one of a matched pair of antibodies for a sandwhich ELISA assay.
Related Products
Anti-Human Luteinizing Hormone (LH) Beta Purified (Leinco Prod. No.: L100)
Anti-Human Luteinizing Hormone (LH) Beta Purified (Leinco Prod. No.: L101)
Anti-Human Luteinizing Hormone (LH) Beta Horseradish Peroxidase (Leinco Prod. No.: H120)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
