产品编号:V103
产品描述:Anti-Human VEGF
反应种属:human
实验方法:ELISA, IHC-F, N, WB
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Vascular Endothelial Growth Factor (VEGF)
Purified No Carrier Protein
(VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen)
Monoclonal Antibody
Prod. No.: V103
Clone: 26503
Isotype: Mouse IgG2b
Pkg. Size: 1mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Vascular Endothelial Growth Factor (VEGF) (Clone 26503) recognizes an epitope on Human VEGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6
Host Species
Mouse
Immunogen
Sf 21-Derived Recombinant Human VEGF165
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), N, WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human VEGF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human VEGF (at 10 ng/ml) is 0.04 - 0.08 µg/ml.
Western Blotting: To detect Human VEGF this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human VEGF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Folkman J et al. (2008) FASEB J. 22: 3728
2. Goodsell DS et al. (2002) The Oncologist 7: 569
3. Mugishima H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh L et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh L et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis LM et al. (2005) J Clin Oncol. 23: 1011
Related Products
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3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
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Recombinant Human VEGF 165 (Leinco Prod. No.: V101)
Human VEGF ELISA Development Kit (Leinco Prod. No.: V107)
Mouse VEGF ELISA Development Kit (Leinco Prod. No.: V167)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
