产品编号:T427
产品描述:Anti-Human TNF-α
反应种属:human
实验方法:ELISA, N, WB, ICC
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Tumor Necrosis Factor Alpha (TNF-α)
Purified No Carrier Protein
(TNFSF1A, TNFSF2, Cachectin, Differentiation-Inducing Factor [DIF], Necrosin, Cytotoxin)
Monoclonal Antibody
Prod. No.: T427
Clone: 1825
Isotype: Mouse IgG1
Pkg. Size: 1mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Tumor Necrosis Factor Alpha (TNF-α) (Clone 1825) recognizes an epitope on Human TNF-α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis.
Known Species Cross Reactivity: Ms-, Hu+, Rt-
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human TNF-α
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human TNF-α is approximately 0.3 ng/well.
Neutralization: This antibody is useful for neutralization of Human TNF-α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TNF-α (at 0.25 ng/ml) is 0.02 - 0.04 µg/ml.
Western Blotting: To detect Human TNF-α this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TNF-α is approximately 5 ng/lane and 100 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 135
2. Adolf GR et al. (1990) Infec Immun. 58: 3996
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T149)
Anti-Mouse TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T188)
Recombinant Human TNF-α (Leinco Prod. No.: T153)
Recombinant Mouse TNF-α (Leinco Prod. No.: T156)
Anti-Mouse TNF-α - Low Endotoxin Functional Formulation (Leinco Prod. No.: T258)
Anti-Mouse TNFα-Purified (Leinco Prod. No.: T259)
Anti-Mouse TNFα-R-phycoerythrin (Leinco Prod. No.: T262)
Human TNF-α ELISA Development Kit (Leinco Prod. No.: T200)
Recombinant Mouse TNF-α (Leinco Prod. No.: T376)
Anti-Rat TNF-α (Leinco Prod. No.: T442)
Anti-Porcine TNF-α (Leinco Prod. No.: T455)
Anti-Porcine TNF-α (Leinco Prod. No.: T456)
Anti-Rat TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T189)
Anti-Human TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T146)
Anti-Human TNF-α (Monoclonal Antibody) (Leinco Prod. No.: T190)
Anti-Human TNF-α-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T187)
Anti-Rat TNF-α (Antigen Aff. Pur.) (Leinco Prod. No.: T150)
Recombinant Rat TNF-α (Leinco Prod. No.: T157)
Anti-Human Extracellular TNF-α-Fluorescein (FITC) (Leinco Prod. No.: T505)
Rat TNF-α ELISA Development Kit (Leinco Prod. No.: T518)
Mouse TNF-α ELISA Development Kit (Leinco Prod. No.: T519)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
