产品编号:T216
产品描述:Anti-Human CCL25
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Chemokine (C-C Motif) Ligand 25 (CCL25)
Purified No Carrier Protein
(SCYA25, TECK, Ckb15)
Monoclonal Antibody
Prod. No.: T216
Clone: 52513
Isotype: Mouse IgG2b
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Chemokine (C-C Motif) Ligand 25 (CCL25) (Clone 52513) recognizes an epitope on Human CCL25. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed ChemoKine). CCL25 is believed to play a role in the development of T-cells.1 It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 is expressed in the small intestinal epithelium and plays an important role in mediating lymphocyte recruitment to this site.2 CCL25 is also highly expressed in the thymus, where it binds to its receptor CCR9 on thymocytes promoting migration and activation.3
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL25
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human TECK is approximately 10 ng/well.
Neutralization: This antibody is useful for neutralization of Human TECK bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TECK (at 3.0 ng/ml) is 3 - 12 µg/ml.
Western Blotting: To detect Human TECK this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human TECK is 10 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Vacari et al. (1997) Immunity 7: 291
2. Agace W et al. (2006) J Immunol. 176: 3642
3. Teixidó J. et al. (2007) J Leukoc Biol. 82: 380
Related Products
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TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL25 (Antigen Aff. Pur.) (Leinco Prod. No.: T167)
Recombinant Mouse TECK (Leinco Prod. No.: T396)
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Anti-Mouse CCL25-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T491)
Recombinant Human CCL25 (Leinco Prod. No.: T165)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
