产品编号:S499
产品描述:Anti-Human SCF
反应种属:human
实验方法:ELISA
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Stem Cell Factor (SCF)
Purified No Carrier Protein
(KITLG, Steel Factor (SF), DKFZp686F2250, KL-1, Kitl, Mast Cell Growth Factor (MGF))
Monoclonal Antibody
Prod. No.: S499
Clone: 13302
Isotype: Mouse IgG1
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Stem Cell Factor (SCF) (Clone 13302) recognizes an epitope on Human SCF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor1 is a paracrine regulator of germ cell development.2 SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.3 SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis4 and liver regeneration.5 SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.6
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human SCF
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, WB
Western Blotting: To detect Human SCF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human SCF is 100 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Ian R et al. (2008) Culture of Animal Cells: A manual of basic technique
2. Toppari J et al. (1999) Endocrin 140: 1492
3. Adamson JW et al. (1991) PNAS 88: 7420
4. Hervé Lejeune et al. (2005) J of Endocrin. 186: 131
5. Ramadori G et al. (2009) Lab Invest.
6. Phan SH et al. (2009) AM J Pathol. 174: 390
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Products are for research use only. Not for use in diagnostic or therapeutic procedures
