产品编号:N139
产品描述:Anti-Human CXCL7
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Chemokine (C-X-C Motif) Ligand 7 (CXCL7)
Purified No Carrier Protein
(PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1)
Monoclonal Antibody
Prod. No.: N139
Clone: 59418
Isotype: Mouse IgG1
Pkg. Size: 1mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Chemokine (C-X-C Motif) Ligand 7 (CXCL7) (Clone 59418) recognizes an epitope on Human CXCL7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.1 It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.2,3
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CXCL7
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
Neutralization: This antibody is useful for neutralization of Human NAP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human NAP-2 (at 1 ng/ml) is 15 - 20 µg/ml.
Western Blotting: To detect Human NAP-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human NAP-2 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Poncz M et al. (1991) J Biol Chem. 266: 5785
2. Walz D et al. (1983) Proc Natl Acad Sci USA 80: 765
3. Carter-Su C et al. (1985) Biochemistry 24: 1762
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CXCL7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N124)
Anti-Human CXCL7 (Antigen Aff. Pur.) (Leinco Prod. No.: N112)
Recombinant Mouse CXCL7 (Leinco Prod. No.: T380)
Recombinant Rat CXCL7 (Leinco Prod. No.: T381)
Anti-Mouse CXCL7 (Leinco Prod. No.: C1380)
Anti-Mouse CXCL7 (Antigen Aff. Pur.) (Leinco Prod. No.: C1478)
Anti-Mouse CXCL7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1523)
Recombinant Mouse Thymus Chemokine-1 (Leinco Prod. No.: T530)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
