产品编号:M152
产品描述:Anti-Human MCP-1
反应种属:human
实验方法:ELISA
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Monocyte Chemotactic Protein-1 (MCP-1)
Purified No Carrier Protein
(CCL2, GDCF-2, GDCF-2 HC11, HC11, HSMCR30, MCAF, MGC9434, SCYA2, SMC-CF, JE)
Monoclonal Antibody
Prod. No.: M152
Clone: 24822
Isotype: Mouse IgG1
Pkg. Size: 1mg, 500ug
Storage: -20°C
Description
Specificity:
Mouse Anti-Human Monocyte Chemotactic Protein-1 (MCP-1) recognizes Human MCP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Monocyte chemotactic protein-1 (MCP-1) is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.4
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL2
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human MCP-1 is approximately 6 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human MCP-1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MCP-1 (at 0.15 ng/ml) is 0.9 - 4.5 µg/ml.
Western Blotting: To detect Human MCP-1 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rhCCL2 is approximately 5 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Yoshimura T. et al. (1989) J. Exp. Med. 169: 1449
2. Oppenheim J.J. et al. (1989) J. Exp. Med. 169: 1485
3. Rollins B.J. et al. (1997) Blood 90: 909
4. Benjamin EJ. et al. (2005) Ciculation 112: 1113
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human MCP-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M122)
Anti-Rat MCP-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M123)
Anti-Mouse MCP-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: J102)
Anti-Rat MCP-1 (Antigen Aff. Pur.) (Leinco Prod. No.: M150)
Anti-Mouse MCP-1 (Antigen Aff. Pur.) (Leinco Prod. No.: J101)
Anti-Human MCP-1 (Antigen Aff. Pur.) (Leinco Prod. No.: M142)
Recombinant Mouse MCP-1 (Leinco Prod. No.: J100)
Recombinant Human MCP-1 (Mucin) (Leinco Prod. No.: M1256)
Anti-Human MCP-1 (Leinco Prod. No.: M1271)
Recombinant Human MCP-1 (Leinco Prod. No.: M156)
Recombinant Rat MCP-1 (Leinco Prod. No.: M164)
Mouse MCP-1 ELISA Development Kit (Leinco Prod. No.: J104)
Human MCP-1 ELISA Developmant Kit (Leinco Prod. No.: M1109)
Rat MCP-1 ELISA Development Kit (Leinco Prod. No.: M1323)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
