产品编号:C1481
产品描述:Anti-Human CCL16
反应种属:human
实验方法:ELISA, IHC, FC, N, WB
标记:
规格:100ug
供应商:leinco
价格(RMB):5325.00
订购:
说明书:
Goat Anti-Human Chemokine (C-C motif) Ligand 16 (CCL16)
Antigen Affinity Purified
(LEC, NCC-4, HCC-4, LMC, LCC-1, MTN-1, IL-10-Inducible Chemokine, SCYA16, SCYL4, CKb12)
Polyclonal Antibody
Prod. No.: C1481
Host: Goat
Pkg. Size: 1mg, 100ug
Storage: -20°C to -70°C
Description
Specificity:
Goat Anti-Human Chemokine (C-C motif) Ligand 16 (CCL16) recognizes Human CCL16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background:
Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes.1 Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-γ and bacterial lipopolysaccharide. CCL16 is a potent maturation factor for monocyte-derived DCs (MoDCs) through differential use of its four receptors and an indirect regulator of Th cell differentiation.2 CCL16 play a role in hepatic vascular formation during development and in angiogenesis associated to hepatic diseases.3
Immunogen
E. coli-Derived Recombinant Human HCC-4
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human CCL16 is approximately 0.16 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human CCL16 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL16 (at 0.75 ng/ml) is 4.0 - 16.0 µg/ml.
Western Blotting: To detect Human CCL16 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CCL16 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Youn et al.(1998) Biochem. Biophys. Res. Commun. 247: 217
2. Giovarelli M. et al.(2006) J. Immunol. 177: 6143
3. Bussolino F. et al.(2004) Blood 103: 40
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL16 (Antigen Aff. Pur.) (Leinco Prod. No.: L125)
Recombinant Human CCL16 (Leinco Prod. No.: L124)
Anti-Human CCL16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L139)
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Recombinant Human CCL16 (Leinco Prod. No.: H1478)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
