产品编号:I-629
产品描述:Anti-Human IL-1β
反应种属:human
实验方法:ELISA, N, WB, ICC
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Interleukin-1 Beta (IL-1β)
Purified No Carrier Protein
(IL1B, IL-1, IL1-Beta, IL1F2)
Monoclonal Antibody
Prod. No.: I-629
Clone: 8516
Isotype: Mouse IgG1
Pkg. Size: 1mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin-1 beta (IL-1β) (Clone 8516) recognizes an epitope on Human IL-1β. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 5% cross-reactivity with rmIL-1β and rpIL-β and no cross-reactivity with rrIL-1β, rmIL-1α, rhIL-1ra, rmIL-1ra, or rrIL-1α.
Background:
Interleukin 1 beta (IL1 beta) is a proinflammatory cytokine belonging to IL1 family that is synthesized as a precursor. The IL1 beta precursor form is not biologically active. It is expressed mainly by stimulated macrophages and monocytes.1,2 IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. It has been recently found that IL1 beta is a major cause of severe inflammation in the mouse model of Crohns disease which makes IL1 beta an important target for this disease.3
Known Species Cross Reactivity: Hu+, Rt-
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human IL-1β
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), FC, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human IL-1 is approximately 0.6 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 0.5-5.0 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-1. A suggested method would be to stain cells expressing IL-1 with 0.25 µl per 1-5 x 105 cells in a total staining volume of ≤ 200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human IL-1β bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-1β (at 50 pg/ml) is 0.001 - 0.003 µg/ml.
Western Blotting: To detect Human IL-1β this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-1β is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Dinarello CA et al. (1996) Blood 87: 2095
2. Yuan et al. (1993) Cell 75: 641
3. Shin Maeda et al. (2005) Science 4: 735
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Products are for research use only. Not for use in diagnostic or therapeutic procedures
