产品编号:I-639
产品描述:Anti-Human IL-15
反应种属:human
实验方法:ELISA
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Interleukin 15(IL-15)Purified No Carrier Protein
(IL-T, MGC9721)
Monoclonal Antibody
Prod. No.: I-639
Clone: 34593
Isotype: Mouse IgG1
Pkg. Size: 1mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin 15 (IL-15) (Clone 34593) recognizes an epitope on Human IL-15. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhIL-2.
Background:
Interleukin 15 (IL-15) is a novel cytokine that uses beta- and gamma-chain of IL-2R for signal transduction and is structurally similarity to IL-2. IL-15 is secreted by activated monocytes/macrophages following infection by virus.1 This cytokine induces cell proliferation of natural killer cells. This cytokine and interleukin 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. IL-15 can recruit and activate T lymphocytes in the synovial membrane, thereby contributing to Rheumatoid arthritis pathogenesis.2
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-15 >98%
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human IL-15 is approximately 5.0 ng/well.
Neutralization: This antibody is useful for neutralization of Human IL-15 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-15 (at 10 ng/ml) is 0.05-0.1 µg/ml.
Western Blotting: To detect Human IL-15 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-15 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Yoshikai Y.et al. (1996) J. Immunol. 156: 663
2. Liew FY. et al. (1996) Nat. Med. 4: 643
Related Products
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TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures
