产品编号:I-153
产品描述:Anti-Human IL-11
反应种属:human
实验方法:ELISA, N
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Interleukin-11(IL-11)Purified No Carrier Protein
(AGIF(Adipogenesis Inhibitory Factor))
Monoclonal Antibody
Prod. No.: I-153
Clone: 22616
Isotype: Mouse IgG2b
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin-11 (IL-11) (Clone 22616) recognizes an epitope on Human IL-11. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows 100% cross-reactivity with rmIL-11 and no cross-reactivity with rhIL-6, rhIL-6 R, rhLIF, rhOSM, rhCT-1, rhCNTF, or rhCLC.
Background:
Interleukin 11 (IL-11) is a multifunctional cytokine isolated from bone marrow-derived stromal cells. It is a key regulator of multiple events in hemtaopoiesis, most notably the stimulation of megakaryocyte maturation.1 It is also known under the names Adipogenesis inhibitory factor (AGIF)2 and Oprelvekin. IL-11 improves platelet recovery after chemotherapy-induced thrombocytopenia, induces acute phase proteins, modulates antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis.
Host Species
Mouse
Immunogen
Sf 21-Derived Recombinant Human Interleukin-11
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
Neutralization: This antibody is useful for neutralization of Human IL-11 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-11 (at 1.0 ng/ml) is 25 - 50 µg/ml.
Western Blotting: To detect Human IL-11 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-11 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Williams DA et al. (1990) Proc. Natl. Acad. Sci. USA87: 7512
2. Takiguchi Y et al. (1991) FEBS Lett. 283: 199
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human IL-11-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-266)
Anti-Human IL-11 (Antigen Aff. Pur.) (Leinco Prod. No.: I-155)
Recombinant Mouse IL-11 (Leinco Prod. No.: I-561)
Anti-Mouse IL-11-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-774)
Recombinant Human IL-11 (Leinco Prod. No.: I-194)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
