产品编号:I-635
产品描述:Anti-Human IL-10
反应种属:human
实验方法:ELISA
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Interleukin 10 (IL-10)
Purified No Carrier Protein
(CSIF, IL10A, MGC126450, MGC126451, TGIF)
Monoclonal Antibody
Prod. No.: I-635
Clone: 23738
Isotype: Mouse IgG2b
Pkg. Size: 1mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin 10 (IL-10) (Clone 23738) recognizes an epitope on Human IL-10. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In Western blots, this antibody shows approximately 5% cross-reactivity with rmIL-10.
Background:
Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is a homodimer protein produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and the Type 1 T helper cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. It is released by cytotoxic T-cells to inhibit the actions of NK cells during the immune response to viral infection. IL-10 plays a key role in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens.1
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human IL-10
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
N, WB
Neutralization: This antibody is useful for neutralization of Human IL-10 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-10 (at 5 ng/ml) is 0.1 - 0.5 µg/ml.
Western Blotting: To detect Human IL-10 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-10 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Akdis CA. et al. (2009) Mol. Immunol. 46: 622
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Products are for research use only. Not for use in diagnostic or therapeutic procedures
