产品编号:I-176
产品描述:Anti-Human IL-8
反应种属:human
实验方法:ELISA
标记:
规格:500ug
供应商:leinco
价格(RMB):4130.00
订购:
说明书:
Anti-Human Interleukin-8 (IL-8)
Purified No Carrier Protein
(CXCL8, MDNCE, Neutrophil Activating Factor (NAF), NAP-1 Fractalkine Mucin-Like Stalk, 3-10C, AMCF-I, GCP-1, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, AP1, SCYB8, TSG-1, B-ENAP)
Monoclonal Antibody
Prod. No.: I-176
Clone: 6217
Isotype: Mouse IgG1
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin-8 (IL-8) recognizes Human IL-8. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. IL-8 is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Gene of IL-8 is believed to play a role in the pathogenesis of bronchiolitis. While neutrophil granulocytes are the primary target cells of IL-8 there is a relative wide range of cells (endothelial cells, macrophages, mast cells, keratinocytes) responding to this chemokine, too. When first encountering an antigen, the primary cells to encounter it are the macrophages that phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor. Interleukin-8 is often associated with inflammation. As an example, it has been cited as a proinflammatory mediator in psoriasis.1
Host Species
Mouse
Immunogen
Purified Recombinant IL-8 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), FC, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:BAF208 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is Recombinant Human IL-8 (Mucin) (Leinco Prod. No.: I-597).
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human IL-8 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-8 (at 20 ng/ml) is 0.08 - 0.4 µg/ml.
Western Blotting: To detect Human IL-8 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-8 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Haraldsen G et al. (1998) J Exp Med. 188: 1751
Related Products
Recombinant Human IL-8 (Mucin) (Leinco Prod. No.: I-597)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human IL-8 (Antigen Aff. Pur.) (Leinco Prod. No.: I-152)
Anti-Human IL-8-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-260)
Recombinant Human IL-8, CXC Chemokine (77 a.a.) (Leinco Prod. No.: I-191)
Recombinant Human IL-8 (Endothelial Cell-Derived) (Leinco Prod. No.: I-576)
Anti-Porcine IL-8 (Leinco Prod. No.: C1437)
Recombinant Human IL-8 (Leinco Prod. No.: I-190)
Human IL-8 ELISA Development Kit (Leinco Prod. No.: I-827)
Products are for research use only. Not for use in diagnostic or therapeutic pro
