产品编号:I-175
产品描述:Anti-Human IL-7
反应种属:human
实验方法:ELISA, N
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Interleukin-7 (IL-7)
Purified No Carrier Protein
(Lymphopoietin 1, LP-1, pre-B Cell Factor)
Monoclonal Antibody
Prod. No.: I-175
Clone: 7417
Isotype: Mouse IgG1
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin-7 (IL-7) recognizes Human IL-7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Interleukin 7 (IL-7) is a glycoprotein cytokine initially referred to as pre-B cell growth factor because of its growth-promoting activity for cells early in the B lymphocyte lineage. Subsequent to IL-7 gene cloning and expression, IL-7 was also found to influence the activity of cells in the T lineage.1 IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of all cells in the lymphoid lineage (B and T lymphocytes and NK cells). IL-7 is not only a potent grown stimulus for pre-B cells, but also plays a role in T cell activation.2 IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections.3
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-7 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), N, WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human IL-7 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-7 (at 2.5 ng/ml) is 0.4 - 0.8 µg/ml.
Western Blotting: To detect Human IL-7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-7 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Namen AE. et al. (1990) Int J Cell Cloning 1: 168
2. Reed SG. et al. (1989) J Exp Med. 169: 707
3. Prakash S. et al. (2008) Appl. of Biochem Biotechnol. 151: 93
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IL-7 (Leinco Prod. No.: I-189)
Anti-Mouse IL-7 (Antigen Aff. Pur.) (Leinco Prod. No.: I-166)
Anti-Human IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-258)
Anti-Mouse IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-259)
Recombinant Rat IL-7 (Leinco Prod. No.: I-192)
Anti-Human IL-7 (Antigen Aff. Pur.) (Leinco Prod. No.: I-151)
Recombinant Mouse IL-7 (Leinco Prod. No.: I-209)
Human IL-7 ELISA Development Kit (Leinco Prod. No.: I-826)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
