产品编号:E139
产品描述:Anti-Human Eotaxin-2
反应种属:human
实验方法:ELISA
标记:
规格:500ug
供应商:leinco
价格(RMB):4800.00
订购:
说明书:
Anti-Human Eotaxin-2
Purified No Carrier Protein
(CCL24, MPIF-2, CkB-6, SCYA24)
Monoclonal Antibody
Prod. No.: E139
Clone: 61016
Isotype: Mouse IgG1
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Eotaxin-2 recognizes Human Eotaxin-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent.1 Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells.
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL24
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
Neutralization: This antibody is useful for neutralization of Human Eotaxin-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Eotaxin-2 (at 100 ng/ml) is 0.2 - 0.8 µg/ml.
Western Blotting: To detect Human Eotaxin-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Eotaxin-2 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Marco Baggiolini et al. (1997) J. Exp. Med. 185: 2171
Related Products
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TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse Eotaxin-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E144)
Recombinant Human Eotaxin-2 (Leinco Prod. No.: E112)
Anti-Human Eotaxin-2 (Antigen Aff. Pur.) (Leinco Prod. No.: E117)
Anti-Mouse Eotaxin-2 (Antigen Aff. Pur.) (Leinco Prod. No.: E143)
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Anti-Human Eotaxin-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E125)
Recombinant Mouse Eotaxin-2 (Leinco Prod. No.: E129)
Recombinant Mouse Eotaxin-2 (aa 27-119) (Leinco Prod. No.: E307)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
