产品编号:M1103
产品描述:Anti-Human CXCL9
反应种属:human
实验方法:ELISA, FC, N, WB, ICC
标记:
规格:500ug
供应商:leinco
价格(RMB):4875.00
订购:
说明书:
Anti-Human Chemokine (C-X-C Motif) Ligand 9 (CXCL9)
Purified No Carrier Protein
(CMK, Humig, MIG, SCYB9, Crg-10)
Monoclonal Antibody
Prod. No.: M1103
Clone: 49106
Isotype: Mouse IgG1
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Monokine Induced by Interferon-Gamma (MIG) (Clone 49106) recognizes an epitope on Human MIG. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. Mig may be important not only to recruit T cells to peripheral inflammatory sites, but also in some cases to maximize interactions among activated T cells, B cells, and dendritic cells within lymphoid organs to provide optimal humoral responses to pathogens.1
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CXCL9
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), FC, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is Anti-Human CXCL9-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M131) at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is Recombinant Human MIG (CXCL9) (Leinco Prod. No.: M160).
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIG. A suggested method would be to stain cells expressing MIG with 0.25 µg per 1 - 2.5 x 105 cells in a total staining volume of ≤ 200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human MIG bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MIG (at 1 ng/ml) is 15 - 30 µg/ml.
Western Blotting: To detect Human MIG this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human MIG is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Farber JM. et al. (2002) J. Immunol. 169: 1433
Related Products
Anti-Human CXCL9-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M131)
Recombinant Human MIG (CXCL9) (Leinco Prod. No.: M160)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse CXCL9-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1504)
Anti-Human CXCL9 (Antigen Aff. Pur.) (Leinco Prod. No.: M146)
Recombinant Mouse MIG (Leinco Prod. No.: M187)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
