产品编号:M1092
产品描述:Anti-Human CCL22
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22)
Purified No Carrier Protein
(MDC, A-152E5.1, ABCD-1, DC/B-CK, MGC34554, SCYA22, STCP-1)
Monoclonal Antibody
Prod. No.: M1092
Clone: 57226
Isotype: Mouse IgG2b
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22) (Clone 57226) recognizes an epitope on Human CCL22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemoattract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once thes cells have migrated into tissue.4
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL22
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
This lyophilized monoclonal antibody should be stored desiccated at -20°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
ELISA Cap, N, WB
Neutralization: This antibody is useful for neutralization of Human MDC bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MDC (at 10 ng/ml) is 0.6 - 3 µg/ml.
Western Blotting: To detect Human MDC this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human MDC is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Sozzani S. et al. (2001) Eur. J. Immunol. 31: 812
2. Elias CG. et al. (1998) J Immunol. 161: 5027
3. Gray PW. et al. (1997) J Exp Med. 185: 1604
4. Teixeira MM. et al. (2003) J Leuko Biol. 73: 356
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL22-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M129)
Anti-Mouse CCL22-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1112)
Anti-Mouse CCL22 (Antigen Aff. Pur.) (Leinco Prod. No.: M1111)
Recombinant Human CCL22 (Leinco Prod. No.: M171)
Anti-Human CCL22 (Antigen Aff. Pur.) (Leinco Prod. No.: M176)
Mouse CCL22 ELISA Development Kit (Leinco Prod. No.: M1113)
Recombinant Mouse CCL22 (Leinco Prod. No.: M202)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
