产品编号:M1091
产品描述:Anti-Human MCP-4
反应种属:human
实验方法:ELISA, FC, N
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Monocyte Chemotactic Protein-4 (MCP-4)
Purified No Carrier Protein
(CCL13, SCYA13, NCC-1, SCYL1, CKb10)
Monoclonal Antibody
Prod. No.: M1091
Clone: 73506
Isotype: Mouse IgG1
Pkg. Size: 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Monocyte Chemotactic Protein-4 (MCP-4) (Clone 73506) recognizes an epitope on Human MCP-4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Monocyte chemoattractant protein-4 (MCP-4), also know as CCL13, is a member of a distinct, structurally-related subclass of CC chemokines mainly involved in recruitment of eosinphils to inflammatory sites. MCP-4 is a major chemoattractants for eosinophils, basophils monocytes and T lymphocytes. The MCP protein family bind to specific G-protein-coupled receptors, initiating a signal cascade within the cell1,2 MCP-4 can cause joint destruction in rheumatoid arthritis.3
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL13
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, FC, N
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MCP-4. A suggested method would be to stain cells expressing MCP-4 with 0.25 µg per 1 - 2.5 x 105 cells in a total staining volume of ≤ 200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human MCP-4 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MCP-4 (at 2 ng/ml) is 10 - 30 µg/ml.
References
1. Luster AD et al. (1996) J Immunol. 157: 5613
2. Schleimer RP et al. (1997) J Clin Invest. 99: 926
3. S. Momohara et al. (2005) Rheumatology 10: 1093
Related Products
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Anti-Human MCP-4 (Antigen Aff. Pur.) (Leinco Prod. No.: M145)
Anti-Human MCP-4-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M127)
Recombinant Human MCP-4 (Leinco Prod. No.: M159)
Products are for research use only. Not for use in diagnostic or therapeutic proc
