产品编号:M1090
产品描述:Anti-Human MCP-2
反应种属:human
实验方法:ELISA, N, WB
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Monocyte Chemotactic Protein-2 (MCP-2)
Purified No Carrier Protein
(SCYA8, CCL8, HC14, SCYA10, Small Inducible Cytokine A8, Chemokine (C-C Motif) Ligand 8, Monocyte Chemotactic, C-C Motif Chemokine 8)
Monoclonal Antibody
Prod. No.: M1090
Clone: 35509
Isotype: Mouse IgG1
Pkg. Size: 1 mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Monocyte Chemotactic Protein-2 (MCP-2) (Clone 35509) recognizes an epitope on Human MCP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Monocyte chemotactic protein-2 (MCP-2) is a non-glycosylated polypeptide that is a member of the C-C chemokine subfamily and it specifically attracts monocytes, lymphocytes, basophils and eosinophils. It also plays a role in neoplasia and inflammatory host responses. MCP-2 can bind heparin.1
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL8
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
Neutralization: This antibody is useful for neutralization of Human MCP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MCP-2 (at 0.25 ng/ml) is 1 - 4 µg/ml.
Western Blotting: To detect Human MCP-2 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human MCP-2 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. van Damme J. et al. (1998) J. Immunol. 160: 4034
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human MCP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M124)
Anti-Human MCP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: M143)
Anti-Mouse MCP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1008)
Anti-Mouse MCP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: M1007)
Recombinant Human MCP-2 (Leinco Prod. No.: M157)
Recombinant Mouse MCP-2 (Leinco Prod. No.: M1099)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
