产品编号:M153
产品描述:Anti-Human MCP-3
反应种属:human
实验方法:ELISA, FC, N, WB
标记:
规格:500ug
供应商:leinco
价格(RMB):4650.00
订购:
说明书:
Anti-Human Monocyte Chemotactic Protein-3 (MCP-3)
Purified No Carrier Protein
(CCL7, SCYA6, SCYA7, Small Inducible Cytokine A7, CCL7, NC28, Chemokine (C-C Motif) Ligand 7, FIC, MARC, MGC138463, MGC138465)
Monoclonal Antibody
Prod. No.: M153
Clone: 36320
Isotype: Mouse IgG1
Pkg. Size: 1 mg, 500ug
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Monocyte Chemotactic Protein-3 (MCP-3) (Clone 36320) recognizes an epitope on Human MCP-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.1 MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes.
MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.2 MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.3,4,5
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL7
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, FC, N, WB
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MCP-3. A suggested method would be to stain cells expressing MCP-3 with 0.25 µg per 1 - 5 x 105 cells in a total staining volume of ≤200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human MCP-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MCP-3 (at 1.0 ng/ml) is 1.5 - 4.5 µg/ml.
Western Blotting: To detect Human MCP-3 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rhCCL7 is approximately 5 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Van Damme J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535
2. Parmentier M. et al. (1999) Blood 94: 1899
3. Brosnan CF. et al. (1998) J Neuroimmunol. 86: 20
4. Reddigari SR. et al. (1995) Exp. Dermatol. 4: 260
5. Matikainen S. et al. (2001) Cytokine Growth Factor Rev. 12: 171
Related Products
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human MCP-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M125)
Anti-Mouse MCP-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M126)
Anti-Human MCP-3 (Antigen Aff. Pur.) (Leinco Prod. No.: M144)
Anti-Mouse CCL7 (Antigen Aff. Pur.) (Leinco Prod. No.: M175)
Recombinant Human MCP-3 (Leinco Prod. No.: M158)
Recombinant Mouse MCP-3 (Leinco Prod. No.: M134)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
