产品编号:C161
产品描述:Anti-Human Cytokeratin 18,6 Purified
反应种属:human
实验方法:IHC, IHC-F
标记:
规格:200ug
供应商:leinco
价格(RMB):3375.00
订购:
说明书:
Anti-Human Cytokeratin 18,6 Purified
Monoclonal Antibody
Prod. No.: C161
Clone: LP34
Isotype: Mouse IgG1
Conc.: 0.5 mg/ml
Pkg. Size: 200ug
Storage: 2-8°C
Description
Specificity:
Mouse Anti-Human Cytokeratin 18,6 (Clone LP34) recognizes Human Cytokeratin 18,6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Anti-Human Cytokeratin 18, 6 (clone LP34) reacts with the cytokeratin polypeptides 18 and 6, with molecular weights of 45 kDa and 56 kDa, respectively. The antibody does not react with keratins 1, 8, or 19. Anti-Cytokeratin 18, 6 reacts in cryostat sections with epithelial tissues known to contain filamentous keratins. It reacts with single glandular epithelia to stratified squamous epithelia. Anti-Cytokeratin 18,6 may be used in research studies to identify tumors as being of epithelial or mesothelial origin enabling aplastic carcinomas to be distinguished from other non-carcinoma types of tumors.1,2 The antibody may also be used to trace areas of epithelial tissue in mixed tumors.
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Detergent-insoluble fraction of psoriatic human epidermis
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IHC (Frozen)
IHC (NBF/Par.): The use of Anti-Human Cytokeratin 18,6 (Clone LP34) requires protein digestion pre-treatment of paraffin sections e.g. trypsin. In frozen sections the antibody recognises cytokeratin 5, 6 and 18 with equal intensity.
References
1. Moll, W. R. et al. (1982) Cell 31:11
2. Gatter et al. (1982) J. Clin. Path. 35:125
Products are for research use only. Not for use in diagnostic or therapeutic procedures
