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Anti-Human EGFR Internal Domain-Biotin

产品编号:E103

产品描述:Anti-Human EGFR Internal Domain-Biotin

反应种属:human

实验方法:IHC, ELISA.

标记:

规格:0.25mg

供应商:leinco

价格(RMB):4155.00

订购:订购

说明书:

Anti-Human Epidermal Growth Factor Receptor Internal Domain(EGFR)-Biotin
(ErbB-1, HER1)
Monoclonal Antibody
Prod. No.:   E103
Clone:       F4
Isotype:     Mouse IgG1
Conc.:       0.1 mg/ml
Pkg. Size:   0.25 mg
Storage:     2-8°C

Description
Specificity:
Anti-EGFR (clone F4) recognizes the internal domain with an epitope between amino acids 985-996 from the complete EGF receptor sequence of 1206 amino acids. It is thus about 200 residues from the C-terminus but just outside the region of sequence homology shared by the src oncogene family.4

Background:

EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer - although there is some evidence that preformed inactive dimers may also exist before ligand binding. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers. Over expression of the EGF-receptor is a hallmark of squamous cell carcinomas.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
ELISA Det, IHC (NBF/Par.)

References
1. Berger, S. M. et al. (1987) J. of Pathology 152:297
2. Downward, J. et al. (1984) Nature 311:483
3. Gullick, W. J. et al. (1985) EMBO J. 4:2869
4. Gullick, W. J. et al. (1986) Cancer Research 46:285
5. Gullick, W. J. et al. (1991) Br. Med. Bulletin 47:87
Related Products
Anti-Human Epidermal Growth Factor Receptor (EGFR) External Domain-Biotin (Leinco Prod. No.: E101)
Anti-Human Epidermal Growth Factor Receptor (EGFR) External Domain Purified (Leinco Prod. No.: E100)
Anti-Human Epidermal Growth Factor Receptor (EGFR) Internal Domain-Purified (Leinco Prod. No.: E102)

Products are for research use only. Not for use in diagnostic or therapeutic procedures