产品编号:HIV-139-100ug

产品描述:Recombinant HIV-2 gp36 (390-702)

反应种属:

实验方法:

标记:

规格:100µg

供应商:Prospec

价格(RMB):2100.00

订购:

说明书:

HIV-2 gp-36 (390-702 a.a.)
HIV-2 gp36 (390-702 a.a.) Recombinant
Catalog No. HIV-139

Introduction:
HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids. Immunodeficiency develops more slowly with HIV-2.
HIV-2 is less infectious in the early stages of the virus than with HIV-1.
The infectiousness of HIV-2 increases as the virus progresses.
Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anchored proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion.
HIV-1 gp41 helical regions can form more stable 6-helix bundles than HIV-2 gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25°C), does not require Ca2+ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition.

Description:
HIV-2 gp36 34 kDa recombinant- contains the sequence of HIV-2 envelope immunodominant regions gp36, amino acids 390-702. The protein is fused to beta-galactosidase (114 kDa) at N-terminus.

Source:
Escherichia Coli.

Physical Appearance:
Sterile filtered colorless clear solution.

Formulation:
0.01M Na2CO3, 0.01M Na3EDTA, 0.014 Mβ-mercaptoethanol, 0.02% Sarcosyl.

Purity:
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.

Stability:
Protein should be stored at 4°C.
Refrigerate Upon arrival. DO NOT FREEZE.

Specificity:
Immunoreactive with all sera of HIV-2 infected individuals

Applications:
HIV-2 gp-36 antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.