产品编号:HIV-128-50ug

产品描述:Recombinant HIV-1 TAT Biotin Labeled

反应种属:

实验方法:

标记:

规格:50µg

供应商:Prospec

价格(RMB):14000.00

订购:

说明书:

HIV-1 TAT, Biotin
HIV-1 TAT Recombinant, Biotin Labeled
Catalog No. HIV-128

Introduction:
Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication and infectivity. The HIV-1 TAT is a molecule of 86 aminoacids encoded by two exons. The product of the first exon is sufficient for the transactivation of the HIV-1 promoter. Biotin molecules can be coupled easily to either antigens (such as avidin, streptavidin, Fc binding proteins like Protein A or G) or antibodies and they can be used in a variety of detection systems.
Biotin can be conjugated to many proteins without significantly changing their biological activity. Biotin binds with high affinity to avidin, streptavidin and NeutrAvidin protein.
Every single protein can react with several molecules of biotin, each of which, in turn, can bind a molecule of avidin. This greatly increases the sensitivity of many assay procedures. The avidin-biotin interaction is the strongest known noncovalent biological interaction (Ka = 1015 M-1) between protein and ligand. The bound formation between biotin and avidin is very rapid and, once formed, withstands extremes of pH, organic solvents and other denaturing agents. The avidinbiotin complex can withstand up to 3 M guanidine•HCl. Biotin can be released by 8 M guanidine•HCl at pH 1.5, or by autoclaving.

Description:
HIV-1 TAT Biotin Recombinant- produced in E.coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids encoded by two exons and having chain having a molecular mass of 14kDa and was biotinylated with NHS biotin.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered colorless solution.

Formulation:
1x PBS & 0.05% glycerol.

Purity:
Greater than 90.0% as determined by HPLC analysis & SDS-PAGE.

Stability:
HIV-1 TAT biotin recombinant although stable at 4°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles

Specificity:
Immunoreactive with all sera of HIV-1 infected individuals.

Applications:
HIV-1 TAT Biotin protein biotinylation is determined by Western Blotting and ELISA analysis using streptavidin–HRP conjugated as a detection reagent. Free biotin is eliminated by dialysis against PBS. Protein concentration is determined using a BIORAD protein assay.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.