产品编号:HIV-116-10ug

产品描述:Recombinant HIV-1 TAT Clade-C

反应种属:

实验方法:

标记:

规格:10µg

供应商:Prospec

价格(RMB):1820.00

订购:

说明书:

HIV-1 TAT Clade-C
HIV-1 TAT Clade-C Recombinant
Catalog No. HIV-116

Introduction:
Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication and infectivity. In addition, during acute infection, Tat is released extracellularly by infected cells and is taken up by neighboring cells where it transactivates viral replication and increases virus infectivity.
HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.

Description:
HIV-1 TAT Recombinant- produced in E.coli is a single, non-glycosylated, polypeptide chain containing 100 amino acids encoded by two exons and having chain having a molecular mass of 21 kDa.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
HIV-1 TAT was lyophilized with no additives.

Purity:
Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.

Solubility:
It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized HIV-1 TAT although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 TAT should be stored at 4°C between 2-7 days and for future use below -18°C.
For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles

Specificity:
Immunoreactive with all sera of HIV-1 infected individuals.

Applications:
Recognized by anti-Tat (HIV-1) polyclonal antibody.
Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.