产品编号:HIV-122-100ug

产品描述:Recombinant HIV-1 pol Integrase

反应种属:

实验方法:

标记:

规格:100µg

供应商:Prospec

价格(RMB):2100.00

订购:

说明书:

HIV-1 Integrase
HIV-1 Integrase Recombinant
Catalog No. HIV-122

Introduction:
Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell and is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc fingerdomainwhich is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both Central catalytic domain and C-terminal domains have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimeror a tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process.

Description:
The E.coli derived 26 kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the pol protein (intergrase) and fused with six histidines at the N-terminus.

Source:
Escherichia Coli.

Physical Appearance:
Sterile filtered colorless clear solution.

Formulation:
1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X & 50% Glycerol.

Purity:
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.

Stability:
Protein is shipped at ambient temperature. Upon arrival, Store at
-20°C Stable for Five years frozen. One month in solution at room temperature.

Specificity:
Immunoreactive with all sera of HIV-1 infected individuals.

Applications:
HIV-1 Integrase antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.