产品编号:HIV-124-500ug

产品描述:Recombinant HIV-1 nef

反应种属:

实验方法:

标记:

规格:500µg

供应商:Prospec

价格(RMB):8400.00

订购:

说明书:

HIV-1 nef
HIV-1 nef Recombinant
Catalog No. HIV-124

Introduction:
Human immunodeficiency virus (HIV) is a retrovirus that can lead to a condition in which the immune system begins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the human immune system such as helper T cells (specifically CD4+ T cells), macrophages and dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosis in infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytes that recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genome is converted to double-stranded DNA by a virally encoded reverse transcriptase that is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integrase so that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latent and the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells.

Description:
The E.coli derived 20kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 nef immunodominant regions, 3-190 amino acids.
The HIV-1 nef is fused to beta-galactosidase (114 kDa).

Source:
Escherichia Coli.

Physical Appearance:
Sterile filtered colorless clear solution.

Formulation:
0.01M Na2CO3, 0.01M Na3EDTA and 0.014M B-me.

Purity:
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.

Stability:
Protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.
Stable for Five years frozen. One month in solution at room temperature.

Amino Acid Sequence:
kwskssv igwptvrerm rraepaadgv gaasqdlekh gaitssntaa tnadcawlea
qeeeevgfpv tpqvplrpmt ykaavdlshf lkekgglegl ihsqrrqdil dlwiyhtqgy
fpdwqnytpg pgirypltfg wcyklvpvep ekleeankge ntsllhpvsl hgmddperev
lewrfdsrla.

Specificity:
Immunoreactive with all sera of HIV-1 infected individuals.

Applications:
Antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.