产品编号:HBV-232-100ug

产品描述:Recombinant Hepatitis B Virus Core(1-186)

反应种属:

实验方法:

标记:

规格:100µg

供应商:Prospec

价格(RMB):2100.00

订购:

说明书:

HBV core (1-186 a.a.)
Hepatitis B Virus Core (1-186 a.a.) Recombinant
Catalog No. HBV-232

Introduction:
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.

Description:
The E.coli derived recombinant protein contains the HBV core immunodominant region amino acids 1-186, and fused to a His tag.

Purification Method:
HBV Core protein was purified by proprietary chromatographic technique.

Purity:
HBV Core protein is >90% pure as determined by 10% PAGE (Coomassie staining).

Formulation:
25mM Tris-HCl pH-8.0, 50mM NaCl, 1.5mM EDTA, 1.5mM Urea & 50% glycerol.

Storage:
HBV Core protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.

Stability:
Five years frozen. One month in solution at room temperature.

Specificity:
Immunoreactive with sera HBV-infected individuals.

Applications:
HBV Core antigen is suitablr for ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.