产品编号:520012

产品描述:Microcystins（微囊藻毒素）ELISA Kit

反应种属:

实验方法:

标记:

规格:40T

供应商:abraxis

价格(RMB):4800.00

订购:

说明书:

Test Principles

The Abraxis Microcystin Coated Tube Kit uses a polyclonal antibody that binds
both Microcystins and a Microcystin-enzyme conjugate. Microcystins in the
sample compete with the Microcystin-enzyme conjugate for a limited number of
antibody binding sites. Secondary antibodies (anti-rabbit), which bind anti-
Microcystins, are immobilized to the inside of the test tube. In the assay
procedure you will:
• Add anti microcystin solution and a sample containing Microcystins to the
coated test tube well, followed by Microcystin-enzyme conjugate. The
conjugate competes with any Microcystins in the sample for the same
antibody binding sites.
• Wash away any unbound molecules, after you incubate this mixture for 20
minutes.
• Add clear substrate solution to each well. In the presence of bound
Microcystin-enzyme conjugate, the substrate is converted to a blue
compound. One enzyme molecule can convert many substrate molecules.
Since the same number of antibody binding sites are available in every tube, and
each tube receives the same number of Microcystin-enzyme conjugate molecules,
a sample containing a low concentration of Microcystins allows the antibody to
bind many Microcystin-enzyme conjugate molecules. The result is a dark blue
solution.
Conversely, a high concentration of Microcystins allows fewer Microcystinenzyme
conjugate molecules to be bound by the antibodies, resulting in a lighter
blue solution.