产品编号:ANT-233-500ul

产品描述:Polyclonal Rabbit Anti Glutathione-S-transferase (GST), clone PPGSTSHG

反应种属:

实验方法:WB，IP

标记:

规格:500µl

供应商:ProSpec

价格(RMB):18200.00

订购:

说明书:

GST antibody
Glutathione-S-Transferase, Polyclonal antibody
Catalog No. ANT-233

Type:
Rabbit Antibody Polyclonal

Introduction:
GST family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called 'GST gene fusion system'. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's multiple cloning sites results in expression of a fusion protein - the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions. The GST tag has the size of 220 amino acids, which, compared to other tags like the myc- or the FLAG-tag, is quite big. It is fused to the N-terminus of a protein. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. A GST-tag is often used to separate and purify proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins.

Clone:
PPGSTSHG

Description:
Polyclonal GST is produced by immunizing rabbits with GST protein.

Formulation:
Serum with 0.02% sodium azide.

Stability / Shelf Life:
2 years at -20ºC. 1 month at 4ºC.

Dilution:
Western Blotting: 1:1000.

Applications:
Western Blot, Immunoprecipitation

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.