产品编号:ANT-221-500ul

产品描述:Mouse Anti FLAG peptide HRP

反应种属:

实验方法:WB

标记:

规格:500µl

供应商:ProSpec

价格(RMB):18200.00

订购:

说明书:

FLAG-HRP Antibody
FLAG Peptide, Mouse Antibody Peroxidase Conjugated
Catalog No. ANT-221

Type:
Mouse Antibody Monoclonal

Introduction:
A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.
The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.

Clone:
PFLAGHRPSHG

Description:
Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.

Ig Subclass:
Mouse IgG2b.

Formulation:
1×PBS & 50% glycerol

Stability / Shelf Life:
2 years at -20ºC. 1 month at 4ºC.

Dilution:
Western Blotting: 1:4000.

Applications:
Western Blot

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.