产品编号:L99400163-001

产品描述:COX Polyclonal Antibody - 160103

反应种属:

实验方法:

标记:

规格:1 ea

供应商:Biosense

价格(RMB):4700.00

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COX Polyclonal Antibody

Keywords: Prostaglandin H Synthase; PGHS; Cyclooxygenase

Antigen: purified ovine COX-1 · Host: rabbit · Cross-reactivity: reacts with both COX-1 and -2; affinity for COX-1>COX-2 · Applications: WB and IHC1,2 · Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2 COX-1 is constitutively expressed in almost all animal tissues and is involved in the homeostatic role of eicosanoids.3,4 Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.5 COX-1 and -2 are 70 and 72 kDa proteins, respectively. Both cyclooxygenases have been cloned from a variety of species including human, mouse, rat, and sheep.6,7 COX-1 isoform at the amino acid level, whereas the homology between COX-1 and COX-2 is only about 60%.

 
1  Zheng, D., Fu, S.J., Lysz, T.W., et al. Immunocytochemical localization of cyclooxygenase in the rat lens. Invest Ophthamol Vis Sci 33 178-183 (1992).
2  Pomerantz, K.B., Summers, B., Hajjar, D.P. Eicosanoid metabolism in cholesterol-enriched arterial smooth muscle cells. Evidence for reduced posttranscriptional processing of cyclooxygenase I and reduced cyclooxygenase II gene expression. Biochemistry 32 13624-13635 (1993).
3  Smith, W.L., Marnett, L.J. Prostaglandin endoperoxide synthase: Structure and catalysis. Biochim Biophys Acta 1083 1-17 (1991).
4  Marnett, L.J., Maddipati, K.R. Prostaglandin H synthase. (1991).
5  Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).
6  Funk, C.D., Funk, L.B., Kennedy, M.E., et al. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J 5 2304-2312 (1991).
7  Zhang, V., O’Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).