产品编号:L99400101-001

产品描述:COX-2 Monoclonal Antibody - 160112

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实验方法:

标记:

规格:1ea

供应商:Biosense

价格(RMB):6350.00

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说明书:

COX-2 Monoclonal Antibody

Keywords: PGHS-2; Prostaglandin H Synthase 2; Cyclooxygenase 2

Antigen: human COX-2 amino acids 580-599 (NASSSRSGLDDINPTVLLKE)1 · Host: mouse, clone CX229 · Cross-reactivity: (+) human, ovine, and monkey COX-22 (-) murine, rat, and rabbit COX-2 and COX-1 (all species) · Applications: WB, IHC, and ICC3,4,5,6,7,8,9 · Isotype: IgG1 · Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.10 COX-2 is a 72kDa protein which has been cloned from a variety of species including human, mouse, rat, and sheep. 1,11,12,13 

1  Hla, T., Neilson, K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).
2  Duffy, D.M., Stouffer, R.L. The ovulatory gonadotrophin surge stimulates cyclooxygenase expression and prostaglandin production by the monkey follicle. Molecular Human Reproduction 7(8) 731-739 (2001).
3  Créminon, C., Habib, A., Maclouf, J., et al. Differential measurement of constitutive (COX-1) and inducible (COX-2) cyclooxygenase expression in human umbilical vein endothelial cells using specific immunometric enzyme immunoassays. Biochim Biophys Acta 1254 341-348 (1995).
4  Pang, L., Knox, A.J. Effect of interleukin-1β, tumour necrosis factor-α and interferon-γ on the induction of cyclo-oxygenase-2 in cultured human airway smooth muscle cells. Br J Pharmacol 121 579-587 (1997).
5  Saukkonen, K., Nieminen, O., van Rees, B., et al. Expression of cyclooxygenase-2 in dysplasia of the stomach and in intestinal-type gastric adenocarcinoma. Clinical Cancer Research 7 1923-1931 (2001).
6  Ristimäki, A., Sivula, A., Lundin, J., et al. Prognostic significance of elevated cyclooxygenase-2 expression in breast cancer. Cancer Res 62 632-635 (2002).
7  Ristimäki, A., Nieminen, O., Saukkonen, K., et al. Expression of cyclooxygenase-2 in human transitional cell carcinoma of the urinary bladder. Am J Pathol 158 849-853 (2001).
8  Uotila, P., Valve, E., Martikainen, P., et al. Increased expression of cyclooxygenase-2 and nitric oxide synthase-2 in human prostate cancer. Urology Research 29 25-28 (2001).
9  Rajnakova, A., Moochhala, S., Goh, P.M.Y., et al. Expression of nitric oxide synthase, cyclooxygenase, and p53 in different stages of human gastric cancer. Cancer Lett 172 177-185 (2001).
10  Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).
11  Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).
12  Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).
13  Zhang, V., O’Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).