产品编号:L54000401-096

产品描述:Organophosphate/Carbamate ( OP/C ) ELISA Plate Kit

反应种属:

实验方法:

标记:

规格:96 wells

供应商:Biosense

价格(RMB):7375.00

订购:

说明书:

Organophosphate/Carbamate ( OP/C ) ELISA Plate Kit

Intended Use
For the detection of a wide range of organophosphate including thiophosphate, and carbamate pesticides in water, (drinking water, ground water, surface water and well water). This plate assay can also be used for testing collected dislodgeable residues from a surface wash, as well as pesticide residues prepared as a dried extract (please contact Biosense technical support for information).

Principle
The test is a qualitative, colorimetric assay (modification of the Ellman method) for the detection of organophosphates and carbamates, based on a modification of their inhibition of the enzyme Acetyl Cholinesterase (ACh-E). Ach-E hydrolyzes acetylthiocholine (ATC) which reacts with 5,5’-Dithio-bis(2-Nitrobenzoic Acid) [DTNB] to produce a yellow color which is read at 405 nm. If OP or Carbamate pesticides are present in a sample, they will inhibit ACh-E  reducing or eliminating color formation depending on their concentration. Detection limits of the various OP/C pesticides differ depending on their ability to inhibit the enzyme (refer to Sensitivity table). If it has been established that only a single OP/C is present, the test can be used in conjunction with appropriate standards for quantitative testing

Limitations
The OP/Carbamate Assay will detect organophosphates and carbamates to different degrees. Refer to specificity table for data . The OP/Carbamate Assay kit provides screening results. As with any analytical technique (GC, HPLC, etc...) positive results requiring some action should be confirmed by an alternative method.

For further information please contact us at biosense@biosense.com