产品编号:L35000401-096

产品描述:Cylindrospermopsin ELISA kit

反应种属:

实验方法:

标记:

规格:96 wells

供应商:Biosense

价格(RMB):9760.00

订购:

说明书:

实验原理

The test is a direct competitive ELISA that allows the detection of Cylindrospermopsin. It is based on
the recognition of Cylindrospermopsin by specific antibodies. Cylindrospermopsin, when present in a
sample, and a Cylindrospermopsin-HRP analogue compete for the binding sites of rabbit anticylindrospermopsin
antibodies in solution. The cylindrospermopsin antibodies are then bound by a
second antibody (sheep anti-rabbit) immobilized in the plate. After a washing step and addition of the
substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to
the concentration of the Cylindrospermopsin present in the sample. The color reaction is stopped after a
specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are
determined by interpolation using the standard curve constructed with each run.

• The antibody binds Cylindrospermopsin and does not cross-react with other non-related toxins or compounds.
• The assay range is between 0.05 ppb and 2.0 ppb. The assay sensitivity allows the determination of Cylindrospermopsin in a range of environmental samples         (water, fish tissue, fish plasma, etc).
• Total time for measurement is less than 90 minutes.
• The kit, a 96-well microtiter plate format with ready to use, colour coded reagents, enables simultaneous measurement of multiple samples at a reasonable cost