产品编号:L35000420-096
产品描述:Okadaic Acid (DSP) ELISA kit 冈田酸检测试剂盒
反应种属:
实验方法:
标记:
规格:96 wells
供应商:Biosense
价格(RMB):12200.00
订购:
说明书:
Okadaic Acid (DSP) ELISA kit
We are pleased to introduce the Okadaic Acid (DSP) ELISA kit, in a microtiter plate format. This is a innovative product for rapid, real time mussel tissue testing and compliments the Saxitoxin (PSP) and our ASP (Domoic Acid) ELISA kits.
The Okadaic Acid ELISA is an immunoassay for the quantitative and sensitive detection of Okadaic Acid. Okadaic Acid is one of the toxins associated with diarrheic shellfish poisoning (DSP). This test is suitable for the quantitative and/or qualitative detection of Okadaic Acid in water samples as well as shellfish samples. For shellfish samples a sample preparation is required. If necessary, positive samples can be confirmed by HPLC, GC/MS, or other conventional methods.
Okadaic Acid is one of the “diarrheic shellfish poisons” (DSP) produced by the dinoflagellate species Dinophysis and Prorocentrum. Contamination of shellfish with okadaic acid has been associated with harmful algal blooms throughout the world. In man, DSP causes dose-dependent symptoms of diarrhea, nausea, and vomiting. The action level established by the FDA is 0.2ppm. The EU has established a level of 160ug OA eq (OA, DTXs, PTXs)/kg.
The Okadaic Acid ELISA allows the determination of 40 samples in duplicate determination. Only a few milliliters of sample are required. The test can be performed in less than 2 hours.
Test Principle
The test is a direct competitive ELISA based on the recognition of Okadaic Acid by specific antibodies. Okadaic Acid, when present in a sample, and an okadaic acid-enzyme-conjugate compete for the binding sites of rabbit anti-okadaic acid antibodies in solution. The okadaic acid antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Okadaic Acid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
The Okadaic Acid (DSP) ELISA kit, a 96 well microtiter plate consists of 12 strips of 8 wells, which can be used individually for the test. The standards must be run with each test.
