产品编号:PRO-333-20ug

产品描述:Recombinant Yeast Thioredoxin(TXN1 Yeast)

反应种属:

实验方法:

标记:

规格:20µg

供应商:Prospec

价格(RMB):1820.00

订购:

说明书:

TXN1 Yeast
Thioredoxin Yeast Recombinant
Catalog No. PRO-333

Synonyms:
Thioredoxin-1, Thioredoxin I, TR-I, Thioredoxin-2, TRX1, TRX2, YLR043C.

Introduction:
Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.

Description:
Thioredoxin Yeast Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Lyophilized Powder.

Formulation:
Each mg of protein contains 20mM phosphate buffer pH 7.4.

Solubility:
It is recommended to reconstitute the lyophilized TRX in sterile 18MΩ-cm H2O.

Stability:
TRX although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5).
The specific activity was found to be 3IU/mg.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.