产品编号:PRO-324-50ug

产品描述:Recombinant Human Cardiac Troponin-I（TNI）

反应种属:

实验方法:

标记:

规格:50µg

供应商:Prospec

价格(RMB):1820.00

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说明书:

Catalogue Number  PRO-324 

Synonyms  Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817. 

Introduction  Troponin I (TnI), troponin T (TnT) and troponin C (TnC) form the troponin complex of the thin filaments of striated muscle. TnI is acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains 3 genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). 

Description  Recombinant Human TNI produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 24,016 Dalton.
The TNI is purified by proprietary chromatographic techniques. 

Source  Escherichia Coli. 

Physical Appearance  Sterile Filtered colorless liquid formulation. 

Formulation  TNI solution containing 6M Urea 50mM Tris PH 8. 

Stability  TNI although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. 

Purity  Greater than 98.0% as determined by both(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. 

Protein content  Protein quantitation was carried out by using 0.25 - 5.0 mg/ml Bradford assay vs. BSA. 

Usage  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.