产品编号:PRO-390-5ug

产品描述:Recombinant Human Centromere Protein-B(CENPB)

反应种属:

实验方法:

标记:

规格:5µg

供应商:Prospec

价格(RMB):800.00

订购:

说明书:

CENPB Human
Centromere Protein B Human Recombinant
Catalog No. PRO-390

Synonyms:
Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.

Introduction:
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.

Description:
CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton.
CENPB is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile Filtered liquid formulation.

Formulation:
CENPB is supplied in 20mM HEPES buffer pH-7.6, 250mM sodium chloride, and 20% glycerol.

Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Coating concentration:
0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 90% as determined by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.