产品编号:ALR-002-1000ug
产品描述:Recombinant Soy Bean P34 Protein(SoyBean P34)
反应种属:
实验方法:
标记:
规格:1000µg
供应商:Prospec
价格(RMB):16800.00
说明书:
Soybean P34
Soybean P34 Protein Recombinant
Catalog No. ALR-002
Introduction:
The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.
Description:
The E.Coli derived recombinant protein contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a GST tag at N-terminus.
Method:
Purified by proprietary chromatographic technique.
Purity:
Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Formulation:
50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.
Storage:
Protein is shipped at ambient temperature.
Upon arrival, Store at -20°C
Stability:
Five years frozen. One month in solution at room temperature.
Applications:
Use as an antigen in ELISA and Western Blots.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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